Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2O2 released from bleaching agents

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Apr;109(4):e70-6. doi: 10.1016/j.tripleo.2009.12.020.

Abstract

Objectives: The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture.

Study design: Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay.

Results: In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells.

Conclusions: The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / therapeutic use*
  • Ascorbic Acid / therapeutic use*
  • Carbamide Peroxide
  • Cell Adhesion / drug effects
  • Cell Culture Techniques
  • Cell Line
  • Cell Survival / drug effects
  • Coloring Agents
  • Dentin / drug effects*
  • Dentin Permeability / drug effects
  • Diffusion
  • Humans
  • Hydrogen Peroxide / toxicity*
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Odontoblasts / drug effects
  • Oxidants / toxicity*
  • Peroxides / toxicity
  • Protective Agents / therapeutic use*
  • Spectrophotometry, Ultraviolet
  • Tetrazolium Salts
  • Thiazoles
  • Time Factors
  • Tooth Bleaching*
  • Urea / analogs & derivatives
  • Urea / toxicity

Substances

  • Antioxidants
  • Coloring Agents
  • Oxidants
  • Peroxides
  • Protective Agents
  • Tetrazolium Salts
  • Thiazoles
  • Carbamide Peroxide
  • Urea
  • Hydrogen Peroxide
  • thiazolyl blue
  • Ascorbic Acid